Services

Sequencing Services

The Molecular Genomics Core offers next generation sequencing featuring Illumina's next generation systems. We offer a full suite of services library preparation to generation of sequencing of a wide range of sample types and applications. In addition to featuring full sequencing runs on the HiSeq, NextSeq and MiSeq systems, we offer the flexibility and affordability of splitting sequencing runs with other researchers. By allowing users to share a flowcell with other researchers, we can accomodate people with smaller sample numbers with more cost-effective solutions. Our standard sequencing services include Whole Genome DNA-seq (WGDNA-seq) mRNA-seq, Total RNA-seq, ChIP-seq, ATAC-seq, exome-seq and Whole Genome Bisulfite DNA-seq. We welcome custom sequencing projects. To discuss your project, email us at uscmoleculargenomicscore@usc.edu.

Library Preparation Services

Researchers who elect to have the Core construct their sequencing libraries will enjoy guarantees on minimum sequencing reads (passing filter) for each sample produced (when sequenced at the Core) as well as fast turnaround time compared to customer-prepared libraries.
Library Prep Type Sample Quality Requirements Price
mRNA-Seq High quality RNA $194 / sample
Total RNA-Seq Low to high quality RNA $235 / sample
Single Cell / Ultra Low mRNA-Seq High quality RNA $235 / sample
ChIP-Seq ChIP-treated DNA $160 / sample
Exome-Seq High quality DNA $130 / sample
Whole Genome DNA-Seq High quality DNA $70 / sample
Whole Genome Bisulfite DNA-Seq Low to high quality DNA (genomic or bisulfite converted) Inquire

QC, Quantification and Additional Treatments

Service Price
Ribosomal RNA Cleanup Inquire
Magnetic Bead Cleanup Inquire
Library QC and Quantification Inquire
Sample QC Inquire

Sequencing - Illumina NextSeq500: Flowcell-Sharing Options

The NextSeq flowcell can produce a total of 400 million reads from a flowcell. Often, researchers who only need to sequence a small number samples and / or are under budget constraints would have limited options. With our flowcell sharing option, samples from multiple different users are combined using sample indexing allowing them to use a fraction of a sequencing run. Users have the flexibility of submitting prepared libraries or samples that the Core can construct libraries from (see Library Preparation section for pricing).
Read Length Reads per Sample* Price Turnaround Time**
1 x 75 25 million reads per sample minimum; 25 million read increments $193 / 25 M reads Up to five (5) business weeks
2 x 75 25 million reads per sample minimum; 25 million read increments $221 / 25 M reads Up to five (5) business weeks
1 x 150 25 million reads per sample minimum; 25 million read increments $221 / 25 M reads Up to six (6) business weeks
2 x 150 25 million reads per sample minimum; 25 million read increments $326 / 25 M reads Up to eight (8) business weeks
Libraries constructed by the Core will be guaranteed for delivery of sequencing reads requested (passing filter); customer-provided libraries are not eligible for any guarantees
* Each library tagged with an index is defined as a sample
** Turnaround Time is same for libraries that are made by the Core and for customer-submitted libraries

Sequencing - Illumina NextSeq500: Full Sequencing Run Options

The NextSeq flowcell can produce a total of 400 million reads from a flowcell. Researchers that choose to purchase a full sequencing run will enjoy flexibility to adjust sample numbers and sample read targets to meet their project goals; run more samples at fewer reads per sample or run fewer samples at higher reads per sample. Users have the flexibility of submitting prepared libraries or samples that the Core can construct libraries from (see Library Preparation section for pricing).
Read Length Reads per Sample* Price Turnaround Time**
1 x 75 User defined; total reads must be evenly distributed; divde # samples / 400M reads to determine reads / sample $2,180 Up to four (4) business weeks
2 x 75 User defined; total reads must be evenly distributed; divde # samples / 400M reads to determine reads / sample $3,530 Up to four (4) business weeks
1 x 150 User defined; total reads must be evenly distributed; divde # samples / 400M reads to determine reads / sample $3,530 Up to four (4) business weeks
2 x 150 User defined; total reads must be evenly distributed; divde # samples / 400M reads to determine reads / sample $5,210 Up to four (4) business weeks
Libraries constructed by the Core will be guaranteed for delivery of sequencing reads requested (passing filter); customer-provided libraries are not eligible for any guarantees
* Each library tagged with an index is defined as a sample
** Turnaround Time is same for libraries that are made by the Core and for customer-submitted libraries

Sequencing - Illumina MiSeq: Full Sequencing Run Options

The Core utilizes Illumina's MiSeq v3 kits that produce a total of 25 million reads from a flowcell. The MiSeq is ideal for small genome, microbiota, amplicon, targeted gene and metagenomics sequencing. It is also the only system capable of 2 x 300 (600 cycle) reads.
Read Length Reads per Sample* Price Turnaround Time**
150 cycles User defined; total reads must be evenly distributed; divde # samples / 25 M reads to determine reads / sample $1,590 Up to four (4) business weeks
600 cycles User defined; total reads must be evenly distributed; divde # samples / 25 M reads to determine reads / sample $2,275 Up to four (4) business weeks
Libraries constructed by the Core will be guaranteed for delivery of sequencing reads requested (passing filter); customer-provided libraries are not eligible for any guarantees
** Turnaround Time is same for libraries that are made by the Core and for customer-submitted libraries